2. Development of novel in vivo and in vitro diagnostic tools for neurodegenerative disorders.

 Development of diagnostics for Neurodegenerative disorders face several challenges. The fact that the brain is such an essential, not regenerating and well protected organ limit non-invasive diagnostics to measuring behavior and physiological output, analysis of plasma and cerebrospinal fluid (csf) and neuroimaging including positron emission tomography (PET). Secondly, the majority of neurodegenerative disorders have complex and overlapping pathophysiology including disease specific and more common pathological processes (e.g. neuro-inflammation). Patients would benefit from a more extended pallet of diagnostic tools that could be utilized in the clinic to (early) diagnose ongoing disease processes, stratify patient groups and monitor treatment effects.

Our main focus is to explore the expression and distribution of many suggested targets using antibody-based approaches. These allow us to identify changes in expression but also protein distribution in the brain, blood plasma and csf in relation to ongoing disease processes. To ensure efficient and economic selection of targets to be included and explored, experts in the field of in vivo and in vitro diagnostics teamed up for this project.

For this project we utilize the >45,000 antibodies generated in the human protein atlas project and in house generated antibodies (Atlas Antibodies AB) to explore protein distribution in 1) human brain tissue using immunofluorescence approaches and 2) quantify protein levels in plasma and csf using suspension bead array. Our goal is to identify a single or panel of proteins that could be targets for in vivo or in vitro diagnostics. Develop a dual-binder assay (plasma and csf) and explore the (radio) chemistry for 1-2 PET targets.

 

Principal investigators:  Jan Mulder
Partners: GE Health Care (Rabia Ahmad) and Atlas Antibodies (Caroline Kampf)
Academic Partners: Balazs Gulyas (NTU&KI), Christer Halldin (KI), Tomas Hökfelt (KI), Peter Nillsson (KTH), Jochen Schwenk (KTH), Mathias Uhlén (KTH)
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